CRISPR-Cas9 mediated efficient PD-1 disruption on human primary T cells for adoptive therapy
نویسندگان
چکیده
Methods Here we described for the first time a non-viral mediated approach to reprogram primary human T cells by disruption of PD-1. Results We showed that the gene knockout of PD-1 by electroporation of plasmids encoding sgRNA and Cas9 was technically feasible. The disruption of PD-1 resulted in significant reduction of PD-1 expression but didn’t affect the viability of primary human T cells. Cellular immune response of the gene modified T cells was characterized by up-regulated IFN-g production and enhanced cytotoxicity.
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